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medium  (PromoCell)


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    Structured Review

    PromoCell medium
    Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/airway+epithelial+cell+basal+medium/Airway+Epithelial+Cell+Basal+Medium/pm42234680-177-4-21
    Average 95 stars, based on 85 article reviews
    medium - by Bioz Stars, 2026-09
    95/100 stars

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    Cell Culture:

    Article Title: Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxin.
    Article Snippet: .. PTEC/PBEC were harvested by scraping the cells from the luminal surface using a scalpel blade and cultivated in a T75 collagen I (Merck, Cat. No. C3867)-coated cell culture flask in Airway Epithelial Cell Basal Medium (AEBM; PromoCell, Cat. No. C21260) supplemented with several growth factors and antibiotics (Airway Epithelial Cell Growth Medium, AEGM; [see Table S1 at https://doi.org/10.5281/zenodo.18400453]) at 37°C and 5% CO2 in a humidified atmosphere. .. When cells reached confluence after approximately 5 days, they were detached using 0.05% trypsin-EDTA (TE; stock 0.5%, Thermo Fisher Scientific, Cat. No. 15400054) and were either used for ALI cultures or cryo-preserved in AEGM supplemented with 40% fetal calf serum (FCS; Biochrom, Cat. No. S 0615) and 10% dimethyl sulfoxide (DMSO; Merck, Cat. No. D2650) and stored in liquid nitrogen.

    Article Title: Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxin
    Article Snippet: .. PTEC/PBEC were harvested by scraping the cells from the luminal surface using a scalpel blade and cultivated in a T75 collagen I (Merck, Cat. No. C3867)-coated cell culture flask in Airway Epithelial Cell Basal Medium (AEBM; PromoCell, Cat. No. C21260 ) supplemented with several growth factors and antibiotics (Airway Epithelial Cell Growth Medium, AEGM; [see Table S1 at https://doi.org/10.5281/zenodo.18400453 ]) at 37°C and 5% CO 2 in a humidified atmosphere. .. When cells reached confluence after approximately 5 days, they were detached using 0.05% trypsin-EDTA (TE; stock 0.5%, Thermo Fisher Scientific, Cat. No. 15400054) and were either used for ALI cultures or cryo-preserved in AEGM supplemented with 40% fetal calf serum (FCS; Biochrom, Cat. No. S 0615) and 10% dimethyl sulfoxide (DMSO; Merck, Cat. No. D2650) and stored in liquid nitrogen.



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